Research article

The effect of erythropoietin on healing of obstructive vs nonobstructive left colonic anastomosis: an experimental study

Munevver Moran¹, M Mahir Ozmen^1*, A Polat Duzgun¹, Riza Gok¹, Nurten Renda³, Selda Seckin² and Faruk Coskun¹

• * Corresponding author: M Mahir Ozmen mahir.ozmen@tubitak.gov.tr

Author Affiliations

¹ Department of Surgery, Ankara Numune Teaching and Research Hospital, Ankara, Turkey

² Department of Pathology, Ankara Numune Teaching and Research Hospital, Ankara, Turkey

³ Department of Biochemistry, Hacettepe University Medical School, Ankara, Turkey

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World Journal of Emergency Surgery 2007, 2:13 doi:10.1186/1749-7922-2-13

Published: 15 May 2007

Abstract

Background

Anastomotic leakage is an important problem following primary resection in the left colon and is even more prominent when obstruction is present. We aimed to evaluate the possible effects of erythropoietin on the healing of anastomosis under both obstructive and non-obstructive states.

Methods

Forty male Wistar albino rats were divided into four groups. In group I, two cm left colonic resection and primary anastomosis were done. In group II, left colon were completely ligated and 24 hours later animals were re-operated for segmental resection. The same procedures were performed for rats in group III and IV in respect to group I and II and, 500 IU/kg a day erythropoietin were given in the latter two groups for seven days. For the quantative description of anastomotic healing mechanical, biochemical and histopathological parameters were employed on the seventh day and the animals were sacrificied.

Results

Although erythropoietin had positive effects on bursting pressure in group IV when compared to group II, it has no effect in group III. Despite the increased tissue hydroxyproline levels in group IV, erythropoietin failed to show any effects in group III.

Erythropoietin had positive effects on neovascularization, fibroblast proliferiation and storage of collagen in group IV.

Conclusion

We failed to find any direct and evident effects of erythropoietin on healing of left colonic anastomosis. On the other hand, erythropoietin might prevent negative effects of obstruction on healing.